Evaluation of MYC Gene Amplification in Prostate Cancer Using a Dual Color Chromogenic In Situ Hybridization (Dual CISH) Assay

Abstract

Lerda Daniel, Cabrera Marta, Flores Jorge, Gutierrez Luis, Chierichetti Armando, Paladini Mario, Revol Martin, Garcia Ã?nto Hernan

Objective: The overall purpose of the study was to demonstrate applicability of the DAKO dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, Glostrup) with respect to fluorescence in situ hybridization (FISH) probes MYC-C. Methods: MYC gene amplification by FISH and DAKO dual-color CISH Results: The study showed that the dual-color CISH assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into chromogenic signals. The dual –color CISH assay was performed on 40 cases of prostate cancer. Amplification was identified in 12 of 40 (30%) tumors. No amplification was seen in 28 of 40 (70%) tumors. FISH data were available in total of 40 tumors. All tumors showed concordant results between dual-color CISH and FISH for classifying a tumor as MYC amplified or not amplified. Conclusions: We conclude that dual-color DAKO CISH assay is an accurate method for determining MYC gene a