E-ISSN 2146-8362
 

Short Communication 


False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium

Francesco Rivasi, Luca Roncati, Giuseppe Barbolini, Elisabetta Petrella, Renzo Boldorini.

Abstract
Objective. There are now eight known human herpes viruses (HHV) containing a double-stranded DNA genome: Herpes Simplex Virus 1 (HSV1, HHV1), Herpes Simplex Virus 2 (HSV2, HHV2), Varicella-Zoster Virus (VZV, HHV3), Epstein-Barr Virus (EBV, HHV4), Cytomegalovirus (CMV, HHV5), Herpes Virus 6 (HHV6-a, HHV6-b), Herpes Virus 7 (HHV7) and Herpes Virus 8 (HHV8, KSHV - Kaposi’s sarcoma associated herpes virus).
The highest risk of maternal-fetal HSV transmission is among women who acquire a genital infection during pregnancy. Congenital HHV6 infection occurs in 1% of births, a rate similar to that for CMV infection. Congenital HHV6-a infections are asymptomatic, whereas postnatal HHV6-b infections present with acute febrile states. The purpose of this study was to eliminate false positive results in order to achieve an accurate pathological diagnosis for herpes virus infections to the gestational endometrium.
Methods. The biopsies of three suspected cases of gestational herpetic endometritis were submitted to immunohistochemical tests for HHV1+2, HHV5, HHV6-a, HHV6-b, HHV8 and for other clusters of differentiation (CD4, CD8, CD20) in compliance with the ABC method protocol. Samples from the three cases under study were submitted to a real-time polymerase chain reaction (PCR).
Results. All three cases exhibited false intranuclear immunohistochemical positivity to the antibodies being used. Conversely, all three reactions tested negative with biotin inactivation. The PCR for herpesviridae, which was positive in the known herpetic controls, showed negative in all three cases.
Conclusions. Immunohistochemical investigations of the gestational endometrium (particularly in pregnancies near to term) may yield false results for several herpes viruses, as well as for other immunohistochemical reactions obtained using the ABC method without prior biotin inactivation.

Key words: IMMUNOHISTOCHEMISTRY, FALSE RESULTS, HERPESVIRIDAE, CLUSTER OF DIFFERENTIATION (CD), BIOTIN, GESTATIONAL ENDOMETRIUM


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Francesco Rivasi
Articles by Luca Roncati
Articles by Giuseppe Barbolini
Articles by Elisabetta Petrella
Articles by Renzo Boldorini
on Google
on Google Scholar
Article Statistics
 Viewed: 1498
Downloaded: 470
Cited: 0

REFERENCES
Corey L, Wald A. Maternal and neonatal HSV infections. N Engl J Med 2009; 361:1376-85.

http://dx.doi.org/10.1056/NEJMra0807633

[Pubmed]    [PMC Free Fulltext]   
Morris SR, Bauer HM, Samuel MC, Gallagher D, Bolan G. Neonatal herpes morbidity and mortality in California, 1995-2003. Sex Transm Dis 2008; 35:14-18.

[Pubmed]   
Clarke LM, Duerr A,Yeung KH, Brockman S, Barbosa C, Macasaet M. Recovery of cytomegalovirus and herpes simplex virus from upper and lower genital tract specimens from women with pelvic inflammatory disease. J Infect Dis 1997; 176:286-88.

http://dx.doi.org/10.1086/517268

[Pubmed]   
Pass RF. HHV6 and HHV7: persistence and vertical transmission. J Pediatr 2004; 145:432-35.

http://dx.doi.org/10.1016/j.jpeds.2004.07.023

[Pubmed]   
Habdel–Haq NM, Asmab BI. Human herpes virus 6 (HHV6) infection. Indian J Pediatr 2004; 71:89-96.

http://dx.doi.org/10.1007/BF02725664

Ferrante P, Caldarelli-Stefano R, Omodeo-Zorini E, Vago L, Boldorini R, Costanzi G. PCR detection of JC virus DNA in brain tissue from patients with and without progressive multifocal leukoencephalopathy. J Med Virol 1995; 47:219-225.

http://dx.doi.org/10.1002/jmv.1890470306

[Pubmed]   
Yokoyama S, Kashima K, Inoue S, Daa T, Nakayama I, Moriuchi A. Biotin-containing intranuclear inclusions in endometrial glands during gestation and puerperium. Am J Clin Pathol 1993; 99:13-17.

[Pubmed]   
Sickel JZ, di Sant'Agnese PA. Anaomalus immunostaining of "optically clear" nuclei in gestational endometrium. A potential pitfall in the diagnosis of pregnancy-related herpesvirus infection. Arch Pathol Lab Med 1994; 118:831-33.

[Pubmed]   
Fox H. Biotin-rich nuclear inclusions of gestational endometrium, not herpes infection. Pathol Res Pract 1995; 191:1272.

http://dx.doi.org/10.1016/S0344-0338(11)81142-8

Cooper K, Haffajee Z, Taylor L. Comparative analysis of biotin intranuclear inclusions of gestational endometrium using the APAAP, ABC and the PAP immunodetection systems. J Clin Pathol 1997; 50:153-56.

http://dx.doi.org/10.1136/jcp.50.2.153

[Pubmed]    [PMC Free Fulltext]   
Dakshinamurti K, Mistry SP. Tissue and intracellular distribution of biotin-C-1400H in rats and chicks. J Biol Chem 1963; 238:294-96.

[Pubmed]   
Wood GS, Warnke R. Suppression of endogenous avidin-binding activity in tissue and its relevance to biotin-avidin detection systems. J Histoche. Cytochem 1981; 29:1196-2004.

http://dx.doi.org/10.1177/29.10.7028859

How to Cite this Article
Pubmed Style

Rivasi F, Roncati L, Barbolini G, Petrella E, Boldorini R. False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. J Interdiscip Histopathol. 2014; 2(1): 32-37. doi:10.5455/jihp.20130905124106


Web Style

Rivasi F, Roncati L, Barbolini G, Petrella E, Boldorini R. False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. http://www.ejmjih.com/?mno=37224 [Access: May 25, 2018]. doi:10.5455/jihp.20130905124106


AMA (American Medical Association) Style

Rivasi F, Roncati L, Barbolini G, Petrella E, Boldorini R. False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. J Interdiscip Histopathol. 2014; 2(1): 32-37. doi:10.5455/jihp.20130905124106



Vancouver/ICMJE Style

Rivasi F, Roncati L, Barbolini G, Petrella E, Boldorini R. False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. J Interdiscip Histopathol. (2014), [cited May 25, 2018]; 2(1): 32-37. doi:10.5455/jihp.20130905124106



Harvard Style

Rivasi, F., Roncati, L., Barbolini, G., Petrella, E. & Boldorini, R. (2014) False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. J Interdiscip Histopathol, 2 (1), 32-37. doi:10.5455/jihp.20130905124106



Turabian Style

Rivasi, Francesco, Luca Roncati, Giuseppe Barbolini, Elisabetta Petrella, and Renzo Boldorini. 2014. False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. Journal of Interdisciplinary Histopathology, 2 (1), 32-37. doi:10.5455/jihp.20130905124106



Chicago Style

Rivasi, Francesco, Luca Roncati, Giuseppe Barbolini, Elisabetta Petrella, and Renzo Boldorini. "False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium." Journal of Interdisciplinary Histopathology 2 (2014), 32-37. doi:10.5455/jihp.20130905124106



MLA (The Modern Language Association) Style

Rivasi, Francesco, Luca Roncati, Giuseppe Barbolini, Elisabetta Petrella, and Renzo Boldorini. "False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium." Journal of Interdisciplinary Histopathology 2.1 (2014), 32-37. Print. doi:10.5455/jihp.20130905124106



APA (American Psychological Association) Style

Rivasi, F., Roncati, L., Barbolini, G., Petrella, E. & Boldorini, R. (2014) False Immunohistochemical Results for Herpesviridae and Other Clusters of Differentiation Due To Biotin Intranuclear Inclusions in the Gestational Endometrium. Journal of Interdisciplinary Histopathology, 2 (1), 32-37. doi:10.5455/jihp.20130905124106





Most Viewed Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Differences in the Angiogenesis of Benign and Malignant Surface Epithelial Ovarian Tumors Demonstrated by Microvessel Density and Immunohistochemistry
    Shelly Sehgal, Prashant Goyal, Reena Agarwal, Sompal Singh, Awanindra Kumar, Ruchika Gupta, Vinita Kumar, Dipti Agrawal
    J Interdiscip Histopathol. 2013; 1(3): 145-152
    » Abstract & References » doi: 10.5455/jihp.20130204114930

  • Retroperitoneal Non-functioning Paraganglioma: A Case Report
    Faten Limaiem, Saadia Bouraoui, Sirine Bouslama, Sana Ben Slama, Ahlem Lahmar, Sabeh Mzabi-Regaya
    J Interdiscip Histopathol. 2013; 1(3): 168-171
    » Abstract & References » doi: 10.5455/jihp.20130115114630

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Nonspecific Cecal Ulcer: An Obsolete Jargon
    Duvuru Ram, Vilvapathy Senguttuvan Karthikeyan, Sarath Chandra Sistla
    J Interdiscip Histopathol. 2013; 1(2): 85-88
    » Abstract & References » doi: 10.5455/jihp.20121228043742

  • Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)
    Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano
    J Interdiscip Histopathol. 2013; 1(3): 160-162
    » Abstract & References » doi: 10.5455/jihp.20130128010436

  • Double-Hit Lymphoma Presenting as Primary Renal Lymphoma: A Case Report
    Vikas Mehta, Chad Ellimottil, Jodi Speiser, Milind Velankar, Guliz A Barkan
    J Interdiscip Histopathol. 2013; 1(2): 93-97
    » Abstract & References » doi: 10.5455/jihp.20130121075815

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Most Downloaded
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Small Cell Lung Carcinoma: Better Than Its Reputation? A Population Based Study
    Hans Rostad, Erik Heyerdahl Strom, Helge Scott, Maria Ramnefjell, Kjell Kjellevold
    J Interdiscip Histopathol. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/jihp.20120703032839

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Histopathology of the Liver Following Administration of Artesunate in Adult Wistar Rats
    Felix Monday Onyije, Josiah S Hart
    J Interdiscip Histopathol. 2012; 1(1): 26-29
    » Abstract & References » doi: 10.5455/jihp.20120606081137

  • Stented Vessels: A Challenge for Histological Preparation and Microscopy
    Andrea Nolte, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, Tobias Walker
    J Interdiscip Histopathol. 2013; 1(3): 104-112
    » Abstract & References » doi: 10.5455/jihp.20130306112937

  • Role of HPV Vaccine in the Prevention of Cervical Cancer
    Saleh JA, Yusuph H, Zailani ZB
    J Interdiscip Histopathol. 2013; 1(4): 212-216
    » Abstract & References » doi: 10.5455/jihp.20130119122700

  • Histopathological Effects of Varied Fluoride Concentration on Cerebrum in Albino Rats
    Sajad Hamid, Zaffar Kawoosa, Shahnawaz Hamid, M.A. Mir, Athar Hafiz, Irfan Jan, Fayiza Yaqoob
    J Interdiscip Histopathol. 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jihp.20121105123701

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Most Cited Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052
    Cited : 17 times [Click to see citing articles]

  • Renal Biopsy for Research: An Overview of Russian Experience
    Sergei V. Jargin
    J Interdiscip Histopathol. 2014; 2(2): 88-95
    » Abstract & References » doi: 10.5455/jihp.20140312020838
    Cited : 4 times [Click to see citing articles]

  • Cadmium-Induced Testicular Toxicity, Oxidative Stress and Histopathology in Wistar Rats: Sustained Effects of Polyphenol-Rich Extract of Vernonia Amygdalina (Del.) Leaf
    Christian Eseigbe Imafidon, Olatoye Taiwo Risikat, Bamidele Funminiyi Samuel, Ojo Opeyemi Esther, Ademoye Kehinde Aderonke
    J Interdiscip Histopathol. 2016; 4(3): 54-62
    » Abstract & References » doi: 10.5455/jihp.20160618041629
    Cited : 4 times [Click to see citing articles]

  • The Senescence Process of Internal Thoracic Artery
    Antonio Manenti, Luca Roncati, Giuseppe Barbolini, Francesco Rivasi
    J Interdiscip Histopathol. 2013; 1(2): 58-61
    » Abstract & References » doi: 10.5455/jihp.20121211115233
    Cited : 3 times [Click to see citing articles]

  • Primary Biliary Cirrhosis Overlapping with Autoimmune Hepatitis in an HIV-Infected Patient on Antiretroviral Therapy
    Margaret Rae Caplan, Apurva Trivedi, Mary McLaughlin, Annick Hebou, David Kleiner, Theo Heller, Caryn Morse
    J Interdiscip Histopathol. 2013; 1(5): 270-273
    » Abstract & References » doi: 10.5455/jihp.20130624104921
    Cited : 3 times [Click to see citing articles]

  • Evaluation of Amelotin Expression in Benign Odontogenic Tumors
    Daiana Paula Stolf, Tae-Young Lee, Grace Bradley, Bernhard Ganss
    J Interdiscip Histopathol. 2013; 1(5): 236-245
    » Abstract & References » doi: 10.5455/jihp.20130506045106
    Cited : 2 times [Click to see citing articles]

  • Diffuse Cutaneous Leishmaniasis in an HIV Sero-Positive Patient - A Diagnostic Dilemma
    Kumar U Mahesh, Balasaheb Ramling Yelikar, Ratnakar Madhav Rao Potekar, Palin Aparna, Murthy Keshav
    J Interdiscip Histopathol. 2012; 1(1): 45-47
    » Abstract & References » doi: 10.5455/jihp.20121109060828
    Cited : 2 times [Click to see citing articles]

  • Small Cell Lung Carcinoma: Better Than Its Reputation? A Population Based Study
    Hans Rostad, Erik Heyerdahl Strom, Helge Scott, Maria Ramnefjell, Kjell Kjellevold
    J Interdiscip Histopathol. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/jihp.20120703032839
    Cited : 2 times [Click to see citing articles]

  • Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma
    Urania Skoufi, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, Dimitris Tsironis and Antigony Mitselou
    J Interdiscip Histopathol. 2014; 2(3): 135-144
    » Abstract & References » doi: 10.5455/jihp.20140429121735
    Cited : 2 times [Click to see citing articles]

  • Fine Architectonics and Protein Turnover Rate in Myofibrils of Glucocorticoid-Caused Myopathic Rats
    Priit Kaasik, Maria Umnova, Karin Alev, Anne Selart, Teet Seene
    J Interdiscip Histopathol. 2012; 1(1): 5-10
    » Abstract & References » doi: 10.5455/jihp.20120606081246
    Cited : 2 times [Click to see citing articles]