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Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)

Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano.

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Abstract
Abstract
Objective: Chalkbrood is a fungal disease of honey bee brood caused by Ascosphaera apis. This disease is now found around the world and there are some indications that the incidence has increased in recent years. The diagnosis of Chalkbrood is based on the recognition of typical clinical symptoms and on the identification of the causative agent. Despite the numerous methods available for the detection of A. apis, the diagnosis of this pathology is not always easy. Methods: Between June and July 2011 an increase in mortality of honey bees was observed to some apiaries of the Caserta province (Campania region, southern Italy). Larvae (no. = 50) from brood samples without signs of disease and adult honey bees (no. = 50) from the same colonies were collected and submitted to our laboratory for routine histologic evaluation.Results and Conclusions: We demonstrate that histopathology is a sensitive and specific method for the detection and identification of Ascosphaera apis infection and has the additional advantage of being able to provide a diagnosis in larvae without signs of disease.

Key words: histopathological method, Ascosphaera apis, honey bee.


 
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http://dx.doi.org/10.2307/3758091

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Skou JP. More details in support of the class Ascosphaeromycetes. Mycotaxon 1988; 31 (1): 191–198.

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Vet Med Anim Sci 2014; 2(1): 4.

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How to Cite this Article
Pubmed Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436


Web Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). http://www.ejmjih.com/?mno=31740 [Access: May 21, 2018]. doi:10.5455/jihp.20130128010436


AMA (American Medical Association) Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. 2013; 1(3): 160-162. doi:10.5455/jihp.20130128010436



Vancouver/ICMJE Style

Maiolino P, Carella F, Leva GD, Rinaldi L, Cringoli G, Martano M. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol. (2013), [cited May 21, 2018]; 1(3): 160-162. doi:10.5455/jihp.20130128010436



Harvard Style

Maiolino, P., Carella, F., Leva, G. D., Rinaldi, L., Cringoli, G. & Martano, M. (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). J Interdiscip Histopathol, 1 (3), 160-162. doi:10.5455/jihp.20130128010436



Turabian Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. 2013. Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436



Chicago Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1 (2013), 160-162. doi:10.5455/jihp.20130128010436



MLA (The Modern Language Association) Style

Maiolino, Paola, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, and Manuela Martano. "Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)." Journal of Interdisciplinary Histopathology 1.3 (2013), 160-162. Print. doi:10.5455/jihp.20130128010436



APA (American Psychological Association) Style

Maiolino, P., Carella, F., Leva, G. D., Rinaldi, L., Cringoli, G. & Martano, M. (2013) Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica). Journal of Interdisciplinary Histopathology, 1 (3), 160-162. doi:10.5455/jihp.20130128010436





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