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Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari.

Abstract
Background: Delayed muscle growth and regeneration of skeletal muscle in diabetics is believed to be due to diabetic myopathy because of alteration in the skeletal muscle homeostatis. Since vitamin E is a natural antioxidant and is also important for the integrity of sarcolemma, the present study was designed to explore the muscle regenerative potency of d-δ-tocotrienol-rich fraction (d-δ-TRF) on crushed skeletal muscle in healthy and diabetic rats. Materials and Methods: Diabetes was induced through single subcutaneous injection of alloxan (100 mg/kg). Twenty-four albino rats were divided into four groups; healthy control, diabetic control, healthy treated, and diabetic treated. Treated groups received injections orally, daily (200 mg/kg) for 3 weeks. A horizontal skin incision was made on the shaved right mid-thigh region, by splitting the fascia between gluteus maximus and tensor fascia lata, and gluteus maximus was crushed with Kochers forceps. Skin wound was closed with an absorbable suture. The crushed muscle changes were studied by assessing the histopathological features, histomorphological measurements, and biochemical analyses on 3rd week following induction of injury. One-way ANOVA followed by Tukeys test and Student t-test were used for statistical analysis of data. Results: Results obtained through various methods indicate that the d-δ-TRF treated groups have controlled glycemic status, improved antioxidant capacity, faster revascularization, re-innervation, regeneration of myofibers, and connective tissue remodeling. Conclusion: It is, therefore, concluded that the d-δ-TRF is a beneficial nutritional adjuvant for skeletal muscles structural and functional recovery after crushed injury in both healthy and diabetics.

Key words: Antioxidant, crush, d-δ-tocotrienol-rich fraction, diabetes, muscle, rats


 
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Pubmed Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscipl Histopathol. 2017; 5(2): 36-42. doi:10.5455/jihp.20170419104224


Web Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. http://www.ejmjih.com/?mno=261085 [Access: February 23, 2018]. doi:10.5455/jihp.20170419104224


AMA (American Medical Association) Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscipl Histopathol. 2017; 5(2): 36-42. doi:10.5455/jihp.20170419104224



Vancouver/ICMJE Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscipl Histopathol. (2017), [cited February 23, 2018]; 5(2): 36-42. doi:10.5455/jihp.20170419104224



Harvard Style

Elsy, B., Khan, A. A. & Maheshwari, V. (2017) Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscipl Histopathol, 5 (2), 36-42. doi:10.5455/jihp.20170419104224



Turabian Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. 2017. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. Journal of Interdisciplinary Histopathology, 5 (2), 36-42. doi:10.5455/jihp.20170419104224



Chicago Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. "Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats." Journal of Interdisciplinary Histopathology 5 (2017), 36-42. doi:10.5455/jihp.20170419104224



MLA (The Modern Language Association) Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. "Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats." Journal of Interdisciplinary Histopathology 5.2 (2017), 36-42. Print. doi:10.5455/jihp.20170419104224



APA (American Psychological Association) Style

Elsy, B., Khan, A. A. & Maheshwari, V. (2017) Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. Journal of Interdisciplinary Histopathology, 5 (2), 36-42. doi:10.5455/jihp.20170419104224





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