E-ISSN 2146-8362
 

Original Research 


Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats

Bijo Elsy, Aijaz Ahmed Khan, Veena Maheshwari.

Abstract
Background: Delayed muscle growth and regeneration of skeletal muscle in diabetics is believed to be due to diabetic myopathy because of alteration in the skeletal muscle homeostatis. Since vitamin E is a natural antioxidant and is also important for the integrity of sarcolemma, the present study was designed to explore the muscle regenerative potency of d-δ-tocotrienol-rich fraction (d-δ-TRF) on crushed skeletal muscle in healthy and diabetic rats. Materials and Methods: Diabetes was induced through single subcutaneous injection of alloxan (100 mg/kg). Twenty-four albino rats were divided into four groups; healthy control, diabetic control, healthy treated, and diabetic treated. Treated groups received injections orally, daily (200 mg/kg) for 3 weeks. A horizontal skin incision was made on the shaved right mid-thigh region, by splitting the fascia between gluteus maximus and tensor fascia lata, and gluteus maximus was crushed with Kochers forceps. Skin wound was closed with an absorbable suture. The crushed muscle changes were studied by assessing the histopathological features, histomorphological measurements, and biochemical analyses on 3rd week following induction of injury. One-way ANOVA followed by Tukeys test and Student t-test were used for statistical analysis of data. Results: Results obtained through various methods indicate that the d-δ-TRF treated groups have controlled glycemic status, improved antioxidant capacity, faster revascularization, re-innervation, regeneration of myofibers, and connective tissue remodeling. Conclusion: It is, therefore, concluded that the d-δ-TRF is a beneficial nutritional adjuvant for skeletal muscles structural and functional recovery after crushed injury in both healthy and diabetics.

Key words: Antioxidant, crush, d-δ-tocotrienol-rich fraction, diabetes, muscle, rats


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Bijo Elsy
Articles by Aijaz Ahmed Khan
Articles by Veena Maheshwari
on Google
on Google Scholar
Article Statistics
 Viewed: 571
Downloaded: 220
Cited: 0

How to Cite this Article
Pubmed Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscip Histopathol. 2017; 5(2): 36-42. doi:10.5455/jihp.20170419104224


Web Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. http://www.ejmjih.com/?mno=261085 [Access: October 15, 2018]. doi:10.5455/jihp.20170419104224


AMA (American Medical Association) Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscip Histopathol. 2017; 5(2): 36-42. doi:10.5455/jihp.20170419104224



Vancouver/ICMJE Style

Elsy B, Khan AA, Maheshwari V. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscip Histopathol. (2017), [cited October 15, 2018]; 5(2): 36-42. doi:10.5455/jihp.20170419104224



Harvard Style

Elsy, B., Khan, A. A. & Maheshwari, V. (2017) Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. J Interdiscip Histopathol, 5 (2), 36-42. doi:10.5455/jihp.20170419104224



Turabian Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. 2017. Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. Journal of Interdisciplinary Histopathology, 5 (2), 36-42. doi:10.5455/jihp.20170419104224



Chicago Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. "Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats." Journal of Interdisciplinary Histopathology 5 (2017), 36-42. doi:10.5455/jihp.20170419104224



MLA (The Modern Language Association) Style

Elsy, Bijo, Aijaz Ahmed Khan, and Veena Maheshwari. "Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats." Journal of Interdisciplinary Histopathology 5.2 (2017), 36-42. Print. doi:10.5455/jihp.20170419104224



APA (American Psychological Association) Style

Elsy, B., Khan, A. A. & Maheshwari, V. (2017) Regenerative Potential of D-δ-Tocotrienol Rich Fraction on Crushed Skeletal Muscle of Diabetic Rats. Journal of Interdisciplinary Histopathology, 5 (2), 36-42. doi:10.5455/jihp.20170419104224





Most Viewed Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • Differences in the Angiogenesis of Benign and Malignant Surface Epithelial Ovarian Tumors Demonstrated by Microvessel Density and Immunohistochemistry
    Shelly Sehgal, Prashant Goyal, Reena Agarwal, Sompal Singh, Awanindra Kumar, Ruchika Gupta, Vinita Kumar, Dipti Agrawal
    J Interdiscip Histopathol. 2013; 1(3): 145-152
    » Abstract & References » doi: 10.5455/jihp.20130204114930

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Retroperitoneal Non-functioning Paraganglioma: A Case Report
    Faten Limaiem, Saadia Bouraoui, Sirine Bouslama, Sana Ben Slama, Ahlem Lahmar, Sabeh Mzabi-Regaya
    J Interdiscip Histopathol. 2013; 1(3): 168-171
    » Abstract & References » doi: 10.5455/jihp.20130115114630

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Nonspecific Cecal Ulcer: An Obsolete Jargon
    Duvuru Ram, Vilvapathy Senguttuvan Karthikeyan, Sarath Chandra Sistla
    J Interdiscip Histopathol. 2013; 1(2): 85-88
    » Abstract & References » doi: 10.5455/jihp.20121228043742

  • Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)
    Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano
    J Interdiscip Histopathol. 2013; 1(3): 160-162
    » Abstract & References » doi: 10.5455/jihp.20130128010436

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Double-Hit Lymphoma Presenting as Primary Renal Lymphoma: A Case Report
    Vikas Mehta, Chad Ellimottil, Jodi Speiser, Milind Velankar, Guliz A Barkan
    J Interdiscip Histopathol. 2013; 1(2): 93-97
    » Abstract & References » doi: 10.5455/jihp.20130121075815

  • Most Downloaded
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Histopathology of the Liver Following Administration of Artesunate in Adult Wistar Rats
    Felix Monday Onyije, Josiah S Hart
    J Interdiscip Histopathol. 2012; 1(1): 26-29
    » Abstract & References » doi: 10.5455/jihp.20120606081137

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Stented Vessels: A Challenge for Histological Preparation and Microscopy
    Andrea Nolte, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, Tobias Walker
    J Interdiscip Histopathol. 2013; 1(3): 104-112
    » Abstract & References » doi: 10.5455/jihp.20130306112937

  • Small Cell Lung Carcinoma: Better Than Its Reputation? A Population Based Study
    Hans Rostad, Erik Heyerdahl Strom, Helge Scott, Maria Ramnefjell, Kjell Kjellevold
    J Interdiscip Histopathol. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/jihp.20120703032839

  • Role of HPV Vaccine in the Prevention of Cervical Cancer
    Saleh JA, Yusuph H, Zailani ZB
    J Interdiscip Histopathol. 2013; 1(4): 212-216
    » Abstract & References » doi: 10.5455/jihp.20130119122700

  • Histopathological Effects of Varied Fluoride Concentration on Cerebrum in Albino Rats
    Sajad Hamid, Zaffar Kawoosa, Shahnawaz Hamid, M.A. Mir, Athar Hafiz, Irfan Jan, Fayiza Yaqoob
    J Interdiscip Histopathol. 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jihp.20121105123701

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Most Cited Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052
    Cited : 20 times [Click to see citing articles]

  • Renal Biopsy for Research: An Overview of Russian Experience
    Sergei V. Jargin
    J Interdiscip Histopathol. 2014; 2(2): 88-95
    » Abstract & References » doi: 10.5455/jihp.20140312020838
    Cited : 4 times [Click to see citing articles]

  • Cadmium-Induced Testicular Toxicity, Oxidative Stress and Histopathology in Wistar Rats: Sustained Effects of Polyphenol-Rich Extract of Vernonia Amygdalina (Del.) Leaf
    Christian Eseigbe Imafidon, Olatoye Taiwo Risikat, Bamidele Funminiyi Samuel, Ojo Opeyemi Esther, Ademoye Kehinde Aderonke
    J Interdiscip Histopathol. 2016; 4(3): 54-62
    » Abstract & References » doi: 10.5455/jihp.20160618041629
    Cited : 4 times [Click to see citing articles]

  • Characterization of the Blood Brain Barrier in Pediatric Central Nervous System Neoplasms
    Christopher S Hong, Winson Ho, Martin G Piazza, Abhik Ray-Chaudhury, Zhengping Zhuang, John D Heiss
    J Interdiscip Histopathol. 2016; 4(2): 29-33
    » Abstract & References » doi: 10.5455/jihp.20160623053540
    Cited : 4 times [Click to see citing articles]

  • Fine Architectonics and Protein Turnover Rate in Myofibrils of Glucocorticoid-Caused Myopathic Rats
    Priit Kaasik, Maria Umnova, Karin Alev, Anne Selart, Teet Seene
    J Interdiscip Histopathol. 2012; 1(1): 5-10
    » Abstract & References » doi: 10.5455/jihp.20120606081246
    Cited : 3 times [Click to see citing articles]

  • The Senescence Process of Internal Thoracic Artery
    Antonio Manenti, Luca Roncati, Giuseppe Barbolini, Francesco Rivasi
    J Interdiscip Histopathol. 2013; 1(2): 58-61
    » Abstract & References » doi: 10.5455/jihp.20121211115233
    Cited : 3 times [Click to see citing articles]

  • Primary Biliary Cirrhosis Overlapping with Autoimmune Hepatitis in an HIV-Infected Patient on Antiretroviral Therapy
    Margaret Rae Caplan, Apurva Trivedi, Mary McLaughlin, Annick Hebou, David Kleiner, Theo Heller, Caryn Morse
    J Interdiscip Histopathol. 2013; 1(5): 270-273
    » Abstract & References » doi: 10.5455/jihp.20130624104921
    Cited : 3 times [Click to see citing articles]

  • A Comparative Study of Oral Hamartoma and Choristoma
    Ilana Kaplan, Irit Allon, Benjamin Shlomi, Vadim Raiser, Dror M. Allon
    J Interdiscip Histopathol. 2015; 3(4): 129-134
    » Abstract & References » doi: 10.5455/jihp.20151020122441
    Cited : 3 times [Click to see citing articles]

  • Evaluation of Amelotin Expression in Benign Odontogenic Tumors
    Daiana Paula Stolf, Tae-Young Lee, Grace Bradley, Bernhard Ganss
    J Interdiscip Histopathol. 2013; 1(5): 236-245
    » Abstract & References » doi: 10.5455/jihp.20130506045106
    Cited : 2 times [Click to see citing articles]

  • Diffuse Cutaneous Leishmaniasis in an HIV Sero-Positive Patient - A Diagnostic Dilemma
    Kumar U Mahesh, Balasaheb Ramling Yelikar, Ratnakar Madhav Rao Potekar, Palin Aparna, Murthy Keshav
    J Interdiscip Histopathol. 2012; 1(1): 45-47
    » Abstract & References » doi: 10.5455/jihp.20121109060828
    Cited : 2 times [Click to see citing articles]