E-ISSN 2146-8362
 

Original Research 


A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain

Priya Shirish Joshi, Bhagyalaxmi Praveen Hongal, Neha Shashank Agnihotri, Rutuja Shridhar Kempwade.

Abstract
Background: Trace elements are required in small concentrations as essential active components of biological enzyme systems. They are in the form of metalloenzymes and copper is one such component. Copper metabolism is profoundly altered in neoplastic disease. Copper plays an important role in tumor angiogenesis especially in early stages and is also necessary for endothelial cell activation. Copper content in tissues of oral leukoplakia (OL), oral submucous fibrosis (OSMF), and oral squamous cell carcinoma (OSCC) has not been studied extensively. Hence, we attempted this retrospective study to analyze copper content in the tissues by using rhodamine stain for better understanding of pathogenesis.
Aim and Objectives: To evaluate and compare the copper content and its depth of penetration in tissue sections of diagnosed cases of oral precancer and cancer by using rhodamine staining method.
Materials and Methods: This retrospective analytical study was conducted on histologically diagnosed 10 cases each of OL, OSMF, and OSCC. Ten cases of normal mucosa served as control. The selected cases were subjected to staining with rhodamine for qualitative analysis of copper content.
Results: Paired t-test showed a significant increase in copper content in the study group (P = 0.516) as compared to control group. Krushall–Wallis non-parametric test showed higher mean value of copper content in cases of OSMF (2.00) than the cases of OL (1.20) and OSCC (1.70).
Conclusion: Copper content by rhodamine staining technique was found to be higher in OSMF than OL and OSCC this staining technique can be used as a prognostic indicator for assessment of disease progression.

Key words: Areca nut, Copper, Leukoplakia, Rhodamine, Squamous cell carcinoma, Submucous fibrosis.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Priya Shirish Joshi
Articles by Bhagyalaxmi Praveen Hongal
Articles by Neha Shashank Agnihotri
Articles by Rutuja Shridhar Kempwade
on Google
on Google Scholar
Article Statistics
 Viewed: 932
Downloaded: 385
Cited: 0

REFERENCES
1. Rooban T, Saraswathi TR, George A, Joshua E, Ranganathan K. Cytological study of copper in oral submucous fibrosis. Indian J Dent Res 2004;15:129-32. [Pubmed]   
2. Raja KB, Hazarey VK, Peters TJ, Warnakulasuriya S. Effect of areca nut on salivary copper concentration in chronic chewers. Biometals 2007;20:43-7. [DOI via Crossref]    [Pubmed]   
3. Shetty SR, Babu S, Kumari S, Shetty P, Hegde S, Karikal A. Role of serum trace elements in oral precancer and oral cancer – A biochemical study. J Cancer Res Treat 2013;1:1-3.
4. Nayak AG, Chatra LK, Prashanth S. Analysis of copper and zinc levels in mucosal tissue and serum of oral submucous fibrosis patients. World J Dent 2010;1:75-8. [DOI via Crossref]   
5. Trivedy CR, Warnakulasuriya KA, Peters TJ, Senkus R, Hazarey VK, Johnson NW. Raised tissue copper levels in oral submucous fibrosis. J Oral Pathol Med 2000;29:241-8. [DOI via Crossref]    [Pubmed]   
6. Copper – Rhodamine Method. Staining Manual – Mineral and Pigments. Surgical Pathology – Histology.1-2, downloaded [Last accessed on 27 October 2014]. Available from: http://library.med.utah.edu/ WebPath/HISTHTML/MANUALS/COPPER.PDF
7. Jain S, Scheuer PJ, Archer B, Newman SP, Sherlock S. Histological demonstration of copper and copper-associated protein in chronic liver diseases. J Clin Pathol 1978;31:784-90. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
8. Trivedy C, Warnakulasuriya KA, Tavassoli M, Steingrimsdottir H, Penhallow J, Maher R, et al. p53 aberrations in oral submucous fibrosis and oral squamous cell carcinoma detected by immunocytochemistry and PCRSSCP. J Oral Pathol Med 1998;27:72-7. [DOI via Crossref]    [Pubmed]   
9. Margalioth EJ, Schenker JG, Chevion M. Copper and zinc levels in normal and malignant tissues. Cancer 1983;52:868-72. [DOI via Crossref]   
10. Gupta SK, Shukla VK, Vaidya MP, Roy SK, Gupta S. Serum and tissue trace elements in colorectal cancer. J Surg Oncol 1993;52:172-5. [DOI via Crossref]    [Pubmed]   
11. Tkeshelashvili LK, McBride T, Spence K, Loeb LA. Mutation spectrum of copper-induced DNA damage. J Biol Chem 1991;266:6401-6. [Pubmed]   
12. Murti PR, Warnakulasuriya KA, Johnson NW, Bhonsle RB, Gupta PC, Daftary DK, et al. p53 expression in oral precancer as a marker for malignant potential. J Oral Pathol Med 1998;27:191-6. [DOI via Crossref]    [Pubmed]   
13. Trivedy C, Warnakulasuriya KA, Hazarey VK, Tavassoli M, Sommer P, Johnson NW. The upregulation of lysyl oxidase in oral submucous fibrosis and squamous cell carcinoma. J Oral Pathol Med 1999;28:246-51. [DOI via Crossref]    [Pubmed]   
14. Irons RD, Schenk EA, Lee JC. Cytochemical methods for copper. Semiquantitative screening procedure for identification of abnormal copper levels in liver. Arch Pathol Lab Med 1977;101:298-301. [Pubmed]   
15. Gupta N, Saxena S, Gupta S, Gupta S, Singh V, Yadav J. Role of copper in oral submucous fibrosis: A cytological correlation. Indian J Dent Sci 2010;5:29-32.
16. Varghese I, Sugathan CK, Balasubramoniyan G, Vijayakumar T. Serum copper and zinc levels in premalignant and malignant lesions of the oral cavity. Oncology 1987;44:224-7. [DOI via Crossref]    [Pubmed]   

How to Cite this Article
Pubmed Style

Joshi PS, Hongal BP, Agnihotri NS, Kempwade RS. A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. J Interdiscip Histopathol. 2015; 3(3): 105-109. doi:10.5455/jihp.20150717124034


Web Style

Joshi PS, Hongal BP, Agnihotri NS, Kempwade RS. A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. http://www.ejmjih.com/?mno=188998 [Access: May 21, 2018]. doi:10.5455/jihp.20150717124034


AMA (American Medical Association) Style

Joshi PS, Hongal BP, Agnihotri NS, Kempwade RS. A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. J Interdiscip Histopathol. 2015; 3(3): 105-109. doi:10.5455/jihp.20150717124034



Vancouver/ICMJE Style

Joshi PS, Hongal BP, Agnihotri NS, Kempwade RS. A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. J Interdiscip Histopathol. (2015), [cited May 21, 2018]; 3(3): 105-109. doi:10.5455/jihp.20150717124034



Harvard Style

Joshi, P. S., Hongal, B. P., Agnihotri, N. S. & Kempwade, R. S. (2015) A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. J Interdiscip Histopathol, 3 (3), 105-109. doi:10.5455/jihp.20150717124034



Turabian Style

Joshi, Priya Shirish, Bhagyalaxmi Praveen Hongal, Neha Shashank Agnihotri, and Rutuja Shridhar Kempwade. 2015. A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. Journal of Interdisciplinary Histopathology, 3 (3), 105-109. doi:10.5455/jihp.20150717124034



Chicago Style

Joshi, Priya Shirish, Bhagyalaxmi Praveen Hongal, Neha Shashank Agnihotri, and Rutuja Shridhar Kempwade. "A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain." Journal of Interdisciplinary Histopathology 3 (2015), 105-109. doi:10.5455/jihp.20150717124034



MLA (The Modern Language Association) Style

Joshi, Priya Shirish, Bhagyalaxmi Praveen Hongal, Neha Shashank Agnihotri, and Rutuja Shridhar Kempwade. "A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain." Journal of Interdisciplinary Histopathology 3.3 (2015), 105-109. Print. doi:10.5455/jihp.20150717124034



APA (American Psychological Association) Style

Joshi, P. S., Hongal, B. P., Agnihotri, N. S. & Kempwade, R. S. (2015) A Retrospective Study on Qualitative Assessment of Copper Content in Oral Leukoplakia, Submucous Fibrosis and Squamous Cell Carcinoma with Rhodamine Stain. Journal of Interdisciplinary Histopathology, 3 (3), 105-109. doi:10.5455/jihp.20150717124034





Most Viewed Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Differences in the Angiogenesis of Benign and Malignant Surface Epithelial Ovarian Tumors Demonstrated by Microvessel Density and Immunohistochemistry
    Shelly Sehgal, Prashant Goyal, Reena Agarwal, Sompal Singh, Awanindra Kumar, Ruchika Gupta, Vinita Kumar, Dipti Agrawal
    J Interdiscip Histopathol. 2013; 1(3): 145-152
    » Abstract & References » doi: 10.5455/jihp.20130204114930

  • Retroperitoneal Non-functioning Paraganglioma: A Case Report
    Faten Limaiem, Saadia Bouraoui, Sirine Bouslama, Sana Ben Slama, Ahlem Lahmar, Sabeh Mzabi-Regaya
    J Interdiscip Histopathol. 2013; 1(3): 168-171
    » Abstract & References » doi: 10.5455/jihp.20130115114630

  • Nonspecific Cecal Ulcer: An Obsolete Jargon
    Duvuru Ram, Vilvapathy Senguttuvan Karthikeyan, Sarath Chandra Sistla
    J Interdiscip Histopathol. 2013; 1(2): 85-88
    » Abstract & References » doi: 10.5455/jihp.20121228043742

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Histopathology as diagnostic tool for Ascosphaera apis infection in apparently healthy honey bees (Apis mellifera ligustica)
    Paola Maiolino, Francesca Carella, Giovanna De Leva, Laura Rinaldi, Giuseppe Cringoli, Manuela Martano
    J Interdiscip Histopathol. 2013; 1(3): 160-162
    » Abstract & References » doi: 10.5455/jihp.20130128010436

  • Double-Hit Lymphoma Presenting as Primary Renal Lymphoma: A Case Report
    Vikas Mehta, Chad Ellimottil, Jodi Speiser, Milind Velankar, Guliz A Barkan
    J Interdiscip Histopathol. 2013; 1(2): 93-97
    » Abstract & References » doi: 10.5455/jihp.20130121075815

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Most Downloaded
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052

  • Cytomorphometric Analysis of Oral Premalignant and Malignant Lesions Using Feulgen Stain and Exfoliative Brush Cytology.
    Priya Shirish Joshi, Manasi Sandipak Kaijkar
    J Interdiscip Histopathol. 2013; 1(4): 204-211
    » Abstract & References » doi: 10.5455/jihp.20130214064844

  • P53 Immunohistochemical Staining Patterns in Benign, Premalignant and Malignant Lesions of the Oral Cavity: A Study of 68 Cases
    Pankaj Baweja, Vidya Monappa, Geetha Krishnanand
    J Interdiscip Histopathol. 2013; 1(3): 113-120
    » Abstract & References » doi: 10.5455/jihp.20130121120725

  • Small Cell Lung Carcinoma: Better Than Its Reputation? A Population Based Study
    Hans Rostad, Erik Heyerdahl Strom, Helge Scott, Maria Ramnefjell, Kjell Kjellevold
    J Interdiscip Histopathol. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/jihp.20120703032839

  • Histopathology of the Liver Following Administration of Artesunate in Adult Wistar Rats
    Felix Monday Onyije, Josiah S Hart
    J Interdiscip Histopathol. 2012; 1(1): 26-29
    » Abstract & References » doi: 10.5455/jihp.20120606081137

  • Lateral Periodontal Cyst: A Report of Two Cases with Varying Clinico-Radiological Features
    Preethi Balan, Subhas G Babu, Shishir Shetty, Deepa Dsouza
    J Interdiscip Histopathol. 2012; 1(1): 41-44
    » Abstract & References » doi: 10.5455/jihp.20120808010902

  • Stented Vessels: A Challenge for Histological Preparation and Microscopy
    Andrea Nolte, Theresa Braun, Sven Zur Oven Krockhaus, Adelheid Munz, Ulrich Vogel, Christian Schlensak, Hans Wendel, Tobias Walker
    J Interdiscip Histopathol. 2013; 1(3): 104-112
    » Abstract & References » doi: 10.5455/jihp.20130306112937

  • Role of HPV Vaccine in the Prevention of Cervical Cancer
    Saleh JA, Yusuph H, Zailani ZB
    J Interdiscip Histopathol. 2013; 1(4): 212-216
    » Abstract & References » doi: 10.5455/jihp.20130119122700

  • Histopathological Effects of Varied Fluoride Concentration on Cerebrum in Albino Rats
    Sajad Hamid, Zaffar Kawoosa, Shahnawaz Hamid, M.A. Mir, Athar Hafiz, Irfan Jan, Fayiza Yaqoob
    J Interdiscip Histopathol. 2012; 1(1): 30-34
    » Abstract & References » doi: 10.5455/jihp.20121105123701

  • The Role of CD10 Immunohistochemistry in the Grading of Phyllodes Tumour of The Breast
    Huzlinda Hussin,Jayalakshmi Pailoor, Peh Suat Cheng
    J Interdiscip Histopathol. 2013; 1(4): 195-203
    » Abstract & References » doi: 10.5455/jihp.20130307011432

  • Most Cited Articles
  • Prevalence of Anemia and Associated Risk Factors among Pregnant Women Attending Antenatal Care in Azezo Health Center Gondar Town, Northwest Ethiopia
    Meseret Alem, Bamlaku Enawgaw, Aschalew Gelaw, Tigist Kenaw, Mohamed Seid , Yadesa Olkeba
    J Interdiscip Histopathol. 2013; 1(3): 137-144
    » Abstract & References » doi: 10.5455/jihp.20130122042052
    Cited : 17 times [Click to see citing articles]

  • Renal Biopsy for Research: An Overview of Russian Experience
    Sergei V. Jargin
    J Interdiscip Histopathol. 2014; 2(2): 88-95
    » Abstract & References » doi: 10.5455/jihp.20140312020838
    Cited : 4 times [Click to see citing articles]

  • Cadmium-Induced Testicular Toxicity, Oxidative Stress and Histopathology in Wistar Rats: Sustained Effects of Polyphenol-Rich Extract of Vernonia Amygdalina (Del.) Leaf
    Christian Eseigbe Imafidon, Olatoye Taiwo Risikat, Bamidele Funminiyi Samuel, Ojo Opeyemi Esther, Ademoye Kehinde Aderonke
    J Interdiscip Histopathol. 2016; 4(3): 54-62
    » Abstract & References » doi: 10.5455/jihp.20160618041629
    Cited : 4 times [Click to see citing articles]

  • The Senescence Process of Internal Thoracic Artery
    Antonio Manenti, Luca Roncati, Giuseppe Barbolini, Francesco Rivasi
    J Interdiscip Histopathol. 2013; 1(2): 58-61
    » Abstract & References » doi: 10.5455/jihp.20121211115233
    Cited : 3 times [Click to see citing articles]

  • Primary Biliary Cirrhosis Overlapping with Autoimmune Hepatitis in an HIV-Infected Patient on Antiretroviral Therapy
    Margaret Rae Caplan, Apurva Trivedi, Mary McLaughlin, Annick Hebou, David Kleiner, Theo Heller, Caryn Morse
    J Interdiscip Histopathol. 2013; 1(5): 270-273
    » Abstract & References » doi: 10.5455/jihp.20130624104921
    Cited : 3 times [Click to see citing articles]

  • Evaluation of Amelotin Expression in Benign Odontogenic Tumors
    Daiana Paula Stolf, Tae-Young Lee, Grace Bradley, Bernhard Ganss
    J Interdiscip Histopathol. 2013; 1(5): 236-245
    » Abstract & References » doi: 10.5455/jihp.20130506045106
    Cited : 2 times [Click to see citing articles]

  • Diffuse Cutaneous Leishmaniasis in an HIV Sero-Positive Patient - A Diagnostic Dilemma
    Kumar U Mahesh, Balasaheb Ramling Yelikar, Ratnakar Madhav Rao Potekar, Palin Aparna, Murthy Keshav
    J Interdiscip Histopathol. 2012; 1(1): 45-47
    » Abstract & References » doi: 10.5455/jihp.20121109060828
    Cited : 2 times [Click to see citing articles]

  • Small Cell Lung Carcinoma: Better Than Its Reputation? A Population Based Study
    Hans Rostad, Erik Heyerdahl Strom, Helge Scott, Maria Ramnefjell, Kjell Kjellevold
    J Interdiscip Histopathol. 2012; 1(1): 1-4
    » Abstract & References » doi: 10.5455/jihp.20120703032839
    Cited : 2 times [Click to see citing articles]

  • Association of Claudin-1 with E-Cadherin/Catenin Complex, Microvessel Density (MVD)-Related Markers, and Clinicopathological Features in Colorectal Carcinoma
    Urania Skoufi, Dimitrios L Arvanitis, Litsa Lampri, Elli Ioachim, Jim Koutsogiannis, Christina Skoufi, Dimitris Tsironis and Antigony Mitselou
    J Interdiscip Histopathol. 2014; 2(3): 135-144
    » Abstract & References » doi: 10.5455/jihp.20140429121735
    Cited : 2 times [Click to see citing articles]

  • Fine Architectonics and Protein Turnover Rate in Myofibrils of Glucocorticoid-Caused Myopathic Rats
    Priit Kaasik, Maria Umnova, Karin Alev, Anne Selart, Teet Seene
    J Interdiscip Histopathol. 2012; 1(1): 5-10
    » Abstract & References » doi: 10.5455/jihp.20120606081246
    Cited : 2 times [Click to see citing articles]