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Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma

Wenhua Piao, Wei Wang, Henghui Zhang, Xudong Gao, Liyuan Jin, Jianhui Qu, Zhongxian Xu, Yongping Yang, Zhe Piao.

Cited by (1)

Abstract
Objective: Hypomethylation of long interspersed nucleotide elements 1 (LINE-1) promoter has been reported in many cancer types including pancreatic endocrine tumors, colon cancers, and stomach carcinomas. Hypomethylation of LINE-1 is also frequently observed in hepatocellular carcinoma (HCC). However, it still unknown how LINE-1 is involved in the hepatocarcinogenesis.
Methods: The level of LINE-1 promoter methylation in 28 HCC was detected by a methylation specific polymerase chain reaction (MSP) at CpG site seven of LINE-1 promoter region (gene bank: X58075). A fractional allelic loss (FAL) of these tumors was evaluated by a combination of loss of heterozygosity with microsatellite markers D4S1545, D4S2920, D8S264, D8S1752, D16S498, D16S514, and p53. The associations between the level of LINE-1 hypomethylation status and the clinico-pathological parameters were finally observed. The clinicopathological parameters were included hepatitis B virus (HBV) status, cirrhosis, tumor size, tumor differentiation and FAL. Results: High level of hypomethylation of LINE-1 promoter was found both in the advanced tumors (>3 cm), (7/18, 39%) and in the early tumors (<3 cm), (6/10, 60%). Moreover, the high level of deoxyribonucleic acid hypomethylation at the LINE-1 was found in both poorly differentiated tumors (8/13, 61.5%) and well-differentiated tumors (5/15, 33.3%). The mean value of FAL in 28 HCCs was 0.535. All cases were divided according to FAL score: Low FAL (FAL < 0.535) and high FAL (FAL > 0.535). There were 41% (7/17) tumors with a high level of hypomethylation in the low FAL group and 54.5% (6/11) tumors with a high level of hypomethylation in the
high FAL group. No associations between the level of hypomethylation of LINE-1 and HBV infection, age, sex, and cirrhosis were found. Conclusions: These results are strongly suggested that the hypomethylation of LINE-1 plays a role in the hepatocarcinogenesis; moreover, the hypomethylation of LINE-1 occurs not only in the progression of HCC, but also in the early stage of HCC tumorigenesis.

Key words: Global hypo-methylation, hepatocellular carcinoma, long interspersed nuclear element-1


 
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This Article Cited By the following articles

Tissue- and Serum-Associated Biomarkers of Hepatocellular Carcinoma
Biomarkers in Cancer 2016; 8s1(): BIC.S34413.

1
 

How to Cite this Article
Pubmed Style

Piao W, Wang W, Zhang H, Gao X, Jin L, Qu J, Xu Z, Yang Y, Piao Z. Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. J Interdiscipl Histopathol. 2014; 2(4): 191-196. doi:10.5455/jihp.20141027122054


Web Style

Piao W, Wang W, Zhang H, Gao X, Jin L, Qu J, Xu Z, Yang Y, Piao Z. Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. http://www.ejmjih.com/?mno=164072 [Access: February 23, 2018]. doi:10.5455/jihp.20141027122054


AMA (American Medical Association) Style

Piao W, Wang W, Zhang H, Gao X, Jin L, Qu J, Xu Z, Yang Y, Piao Z. Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. J Interdiscipl Histopathol. 2014; 2(4): 191-196. doi:10.5455/jihp.20141027122054



Vancouver/ICMJE Style

Piao W, Wang W, Zhang H, Gao X, Jin L, Qu J, Xu Z, Yang Y, Piao Z. Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. J Interdiscipl Histopathol. (2014), [cited February 23, 2018]; 2(4): 191-196. doi:10.5455/jihp.20141027122054



Harvard Style

Piao, W., Wang, W., Zhang, H., Gao, X., Jin, L., Qu, J., Xu, Z., Yang, Y. & Piao, Z. (2014) Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. J Interdiscipl Histopathol, 2 (4), 191-196. doi:10.5455/jihp.20141027122054



Turabian Style

Piao, Wenhua, Wei Wang, Henghui Zhang, Xudong Gao, Liyuan Jin, Jianhui Qu, Zhongxian Xu, Yongping Yang, and Zhe Piao. 2014. Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. Journal of Interdisciplinary Histopathology, 2 (4), 191-196. doi:10.5455/jihp.20141027122054



Chicago Style

Piao, Wenhua, Wei Wang, Henghui Zhang, Xudong Gao, Liyuan Jin, Jianhui Qu, Zhongxian Xu, Yongping Yang, and Zhe Piao. "Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma." Journal of Interdisciplinary Histopathology 2 (2014), 191-196. doi:10.5455/jihp.20141027122054



MLA (The Modern Language Association) Style

Piao, Wenhua, Wei Wang, Henghui Zhang, Xudong Gao, Liyuan Jin, Jianhui Qu, Zhongxian Xu, Yongping Yang, and Zhe Piao. "Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma." Journal of Interdisciplinary Histopathology 2.4 (2014), 191-196. Print. doi:10.5455/jihp.20141027122054



APA (American Psychological Association) Style

Piao, W., Wang, W., Zhang, H., Gao, X., Jin, L., Qu, J., Xu, Z., Yang, Y. & Piao, Z. (2014) Hypomethylation of Long Interspersed Nuclear Element-1 is Involved in the Early Tumorigenesis of Hepatocellular Carcinoma. Journal of Interdisciplinary Histopathology, 2 (4), 191-196. doi:10.5455/jihp.20141027122054





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